Kit composition (WLRE8208KIT 48T/Pack)

Composition

Content

1 mL×2 Tubes

150 μL×1Tube

Positive control template

100 μL×1Tube

48T

Specificity is superior to PCR

Liquid, freeze-dried powder, freeze-dried ball

Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the results

Design of primers and probe is simple

It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.

The freeze-dried form has a long storage time and is convenient for transportation

Low requirements on equipment

Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;

Applicable to our WL-16-III and other isothermal fluorescence detector

African Swine Fever Virus Isothermal Detection

Operation procedure and process:

Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix.

Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent.

Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid

Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control

Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap)

Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone.

Result determination